Effect of bull on in vitro sperm capacitation induced by different agents in buffalo species (Bubalus bubalis)

P-103: Effects of In Vitro Selenium Supplementation on Buffalo (Bubalus bubalis) Sperm Quality following Cryopreservation

Background: Artificial insemination is a valuable tool in genetic improvement programs and widely used breeding technique in farm animals. Semen processing and cryopreservation causes considerable damage to sperm DNA, motility apparatus, plasma membrane, acrosomal cap and thus, reduced fertility. Selenium is an integral part of glutathione peroxidase (GSH-Px), an enzyme which protects cell inte...

INFECTIOUS CAUSES OF INFERTILITY IN BUFFALO BULL (Bubalus bubalis)

The artifi cial insemination (AI) technique plays an important role in improvement of conception rate, prevention of sexually transmitted diseases and transmission of genetic material to the next generation. The breeding soundness evaluation (BSE) plays an important role in selection of male buffalo for the said purposes. This evaluation is an effective, inexpensive and easy method for selectio...

Evaluation of lipid changes in buffalo (Bubalus bubalis) spermatozoa during in-vitro capacitation and acrosome reaction

In this study, buffalo spermatozoa were in-vitro capacitated and acrosome reacted using modified Tyrode’s bicarbonatebuffered medium (sp-TALP media). Lipids were extracted from freshly ejaculated, in-vitro capacitated and acrosome reacted buffalo spermatozoa. The total lipid content of freshly ejaculated spermatozoa (171.5±2.90 μg/108 cells), capacitated spermatozoa (128.2±3.29 μg/108 cells) an...

Effect of Leptin on In Vitro Nuclear Maturation and Apoptosis of Buffalo (Bubalus bubalis) Oocyte

P-70: Effect of Xylose on Epididymal Sperm Motility in Water Buffalo (Bubalus bubalis)

Background: In mammalian spermatozoa, motility is one of the most important parameters for evaluating the quality of the semen. From a functional point of view, motility is strongly related to the spermatozoa’s ability to manage its energy status, Thus, modulation of energy status could be translated into significant changes in motility patterns. The aim of the present study was to evaluate xyl...